- NCBI Gene Id:
- Protein Name:
- Alias Symbols:
- LERK-2, efnb1-A, efnb1
- Description of Target:
- RABBIT ANTI EPHRIN B (pTyr298)
- Protein Size (# AA):
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express efnb1.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express efnb1.
- Synthetic phosphotide corresponding to an amino acid sequence within Xenopus Ephrin B, which includes phosphorylated Tyr298.
- Product Format:
- Purified IgG - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for OASA07237
- EPHRIN B
- Polyclonal IgG
- Application Info:
- Western Blot: This product detects a band of approximately 46kDa in rat testes cell lysates.
* For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
- Preservative Stabilisers: 0.09% - Sodium Azide (NaN3)
50% Glycerol - 0.01% - Bovine Serum Albumin
Antiserum Preparation: Antisera to xenopus Ephrin B were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solutions: 10mM Hepes pH7.5
- Target Reference:
- 1. Dufour, A. et al. (2003) Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes. Neuron. 39: 453-465.
2. Oike, Y. et al. (2002) Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. Blood. 100: 1326-1333.
3. Palmer, A. et al. (2002) EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL Phosphatase. Mol. Cell. 9: 725-737.
- Tips Information: