RAB8A Antibody - middle region (AVARP13076_P050)

Catalog No: AVARP13076_P050

WB Suggested Anti-RAB8A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysateRAB8A is supported by BioGPS gene expression data to be expressed in 721_B

RAB8A antibody - middle region (AVARP13076_P050) validated by WB using 1. Human Cervical Cancer Cell Lysate (15ug)
2. Monkey Fibroblast Cell Lysate (15ug)
3. Human Cervical Cancer Cell transfected with mouse Rab8A-GFP (15ug) at 1:1000.

Host: Rabbit
Target Name: RAB8A
Sample Type: Human Fetal Liver
Antibody Dilution: 1.0ug/ml

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RAB8A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 85%; Pig: 100%; Rat: 85%; Sheep: 100%; Zebrafish: 85%
• Peptide Sequence: Synthetic peptide located within the following region: GIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITP
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-RAB8A (AVARP13076_P050) antibody is Catalog # AAP30723 (Previous Catalog # AAPP01380)
• Sample Type Confirmation:

  RAB8A is supported by BioGPS gene expression data to be expressed in 721_B

Target Info

• Reference: Roland,J.T., (2007) Mol. Biol. Cell 18 (8), 2828-2837
• Gene Symbol: RAB8A
• Gene Full Name: RAB8A, member RAS oncogene family
• Alias Symbols: MEL, RAB8
• NCBI Gene Id: 4218
• Protein Name: Ras-related protein Rab-8A
• Description of Target: RAB8A is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable.The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: P61006
• Protein Accession #: NP_005361
• Nucleotide Accession #: NM_005370
• Protein Size (# AA): 207
• Molecular Weight: 24kDa
• Protein Interactions: TFRC; SYTL4; SYTL1; TBC1D17; STAU1; UBC; MDM2; RPA3; RPA2; RPA1; STK4; OPTN; Myo5b; MYO5C; EHD3; EHD1; UBL4A; RAB1B; RAB31; COX5A; RAB11B; RAB7A; SCP2; RRBP1; NDUFA7; IDH3G; SNCA; ELAVL1; Rab8a; RPH3A; RAB3IP; RIMS2; RPH3AL; RABIF; MAP4K2; PDE6D; GDI2; OC