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Datasheets/Manuals | Printable datasheet for RAB8A Antibody - C-terminal region (OABB01444) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Chicken|Human |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Ras-related protein Rab-8A is a protein that in humans is encoded by the RAB8A gene. It is mapped to 19p13.12. The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. RAB8A regulates cilia assembly by targeting and promoting fusion of vesicles near the ciliary membrane. This gene requires CEP290 for centrosome localization and that CEP290 regulates entry of RAB8A into the cilium during assembly of this organelle. RAB8A has been shown to interact with Optineurin and MAP4K2. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Ras-related protein Rab-8A(RAB8A) detection. Tested with WB, IHC-P in Human. |
Gene Symbol | RAB8A |
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Gene Full Name | RAB8A, member RAS oncogene family |
Alias Symbols | MEL;mel transforming oncogene (derived from cell line NK14);mel transforming oncogene (derived from cell line NK14)- RAB8 homolog;mel transforming oncogene (RAB8 homolog);oncogene c-mel;RAB8;ras-associated protein RAB8;ras-related protein Rab-8A. |
NCBI Gene Id | 4218 |
Protein Name | Ras-related protein Rab-8A |
Description of Target | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297). Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656). Plays an important role in ciliogenesis (PubMed:21844891). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (PubMed:27103069). |
Uniprot ID | P61006 |
Molecular Weight | 23668 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "RAB8A Antibody - C-terminal region (OABB01444)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Chicken|Human".
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How long will it take to receive "RAB8A Antibody - C-terminal region (OABB01444)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "RAB8A Antibody - C-terminal region (OABB01444)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RAB8A Antibody - C-terminal region (OABB01444)"?
This target may also be called "MEL;mel transforming oncogene (derived from cell line NK14);mel transforming oncogene (derived from cell line NK14)- RAB8 homolog;mel transforming oncogene (RAB8 homolog);oncogene c-mel;RAB8;ras-associated protein RAB8;ras-related protein Rab-8A." in publications.
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What is the shipping cost for "RAB8A Antibody - C-terminal region (OABB01444)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "RAB8A Antibody - C-terminal region (OABB01444)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "RAB8A Antibody - C-terminal region (OABB01444)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "23668 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RAB8A Antibody - C-terminal region (OABB01444)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RAB8A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RAB8A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RAB8A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RAB8A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RAB8A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RAB8A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.