RAB6A Antibody (OAGA00864)

Catalog No: OAGA00864

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
OAGA00864 diluted at 1:1000

RAB6A antibody detects RAB6A protein by western blot analysis.
A. 50 ?g mouse brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (OAGA00864) dilution: 1:1000

RAB6A antibody detects RAB6A protein by western blot analysis.
A. 50 ?g rat brain cell lysate/extract
12 % SDS-PAGE
RAB6A antibody (OAGA00864) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using RAB6A(OAGA00864) antibody at 1:500 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RAB6A(OAGA00864) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain, rat brain
  Cellular Localization: Golgi apparatus membrane; Lipid-anchor
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 208 of RAB6A (Uniprot ID#P20340)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.63 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RAB6A
• Gene Full Name: Ras-related protein Rab-6A
• Alias Symbols: RAB6
• NCBI Gene Id: 5870
• Protein Name: Ras-related protein Rab-6A
• Uniprot ID: P20340
• Protein Accession #: NP_001230647.1
• Molecular Weight: 24 kDa