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Catalog No: OKEH08546
Size:96T
Price: $725.00
SKU
OKEH08546
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)109-120% (n = 20)108-118% (n = 20)101-112% (n = 20)81-93% (n = 20)
EDTA plasma(n=5)93-105% (n = 20)85-95% (n = 20)109-119% (n = 20)105-114% (n = 20)
heparin plasma(n=5)95-106% (n = 20)82-92% (n = 20)96-104% (n = 20)92-105% (n = 20)
ELISA Kit PrincipleAviva Systems Biology RAB3C ELISA Kit (Human) (OKEH08546) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB3C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB3C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAB3C captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.8% (n = 20)
Mean Inter-assay CV%: <=9.2% (n = 20)
ELISA Kit Component
ComponentAmount
RAB3C Microplate96 Wells (12 x 8 Well strips)
RAB3C Lyophilized Standard2
100X Biotinylated RAB3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Protein transport. Probably involved in vesicular traffic.
::Subcellular Location: Cell membrane Lipid-anchor Cytoplasmic side
::Unigene: Hs.4290
SMR: Q96E17
String: 9606.ENSP00000282878
::KEGG: hsa:115827
MIM: 612829
Pfam: PF00071
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.117ng/mL
SpecificityNatural and recombinant Human Ras-related protein Rab-3C
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRAB3C
Gene Full NameRAB3C, member RAS oncogene family
Alias Symbolsras-related protein Rab-3C.
NCBI Gene Id115827
Protein NameQ96E17
Description of TargetRas-related protein Rab-3C
Uniprot IDhttps://www.uniprot.org/uniprot/Q96E17
Protein Accession #NP_001304844.1
Nucleotide Accession #NM_001317915.1
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