RAB3B ELISA Kit (Human) (OKEH08545)

Catalog No: OKEH08545

Size:96T

Human RAB3B ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	109-119% (n = 20)	101-111% (n = 20)	81-89% (n = 20)	99-109% (n = 20)
  EDTA plasma(n=5)	94-106% (n = 20)	114-126% (n = 20)	109-120% (n = 20)	92-102% (n = 20)
  heparin plasma(n=5)	112-122% (n = 20)	110-119% (n = 20)	104-113% (n = 20)	104-116% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology RAB3B ELISA Kit (Human) (OKEH08545) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB3B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAB3B captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.9% (n = 20)
  Mean Inter-assay CV%: <=7.6% (n = 20)
• ELISA Kit Component:
  • RAB3B Microplate: 96 Wells (12 x 8 Well strips)
  • RAB3B Lyophilized Standard: 2
  • 100X Biotinylated RAB3B Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Protein transport. Probably involved in vesicular traffic.
  Subcellular Location: Cell membrane Lipid-anchor Cytoplasmic side
  Unigene: Hs.123072
  SMR: P20337
  String: 9606.ENSP00000360718
  KEGG: hsa:5865
  MIM: 179510
  Pfam: PF00071
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.118ng/mL
• Specificity: Natural and recombinant Human Ras-related protein Rab-3B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RAB3B
• Gene Full Name: RAB3B, member RAS oncogene family
• Alias Symbols: brain antigen RAB3B;ras-related protein Rab-3B.
• NCBI Gene Id: 5865
• Protein Name: P20337
• Description of Target: Ras-related protein Rab-3B
• Uniprot ID: https://www.uniprot.org/uniprot/P20337
• Protein Accession #: NP_002858.2
• Nucleotide Accession #: NM_002867.3