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Catalog No: OKEH07129
Size:96T
Price: $525.00
SKU
OKEH07129
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology RAB37 ELISA Kit (Human) (OKEH07129) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB37 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB37 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAB37 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 103.5%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <8.2%
Inter-Assay CV <9.5%
ELISA Kit Component
ComponentAmount
RAB37 Microplate96 Wells (12 x 8 Well strips)
RAB37 Lyophilized Standard2 x 5 ng
100X RAB37 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity39.2 pg/mL
SpecificityNatural and recombinant Human Ras-related protein Rab-37
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRAB37
Gene Full NameRAB37, member RAS oncogene family
Alias SymbolsRAB37, member of RAS oncogene family;ras-related protein Rab-37.
NCBI Gene Id326624
Protein NameQ96AX2
Description of TargetRas-related protein Rab-37
Uniprot IDhttps://www.uniprot.org/uniprot/Q96AX2
Protein Accession #NP_001006639
Nucleotide Accession #NM_001006638
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