Catalog No: OCOA20925
Size:20UG
Price: $169.00
SKU
OCOA20925
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RAB35 Protein Lysate (OCOA20925)

Datasheets/ManualsPrintable datasheet for RAB35 Protein Lysate (OCOA20925)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionRAB35 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolRAB35
Gene Full NameRAB35, member RAS oncogene family
Alias SymbolsGTP-binding protein RAY;H-ray;RAB1C;Ras-related protein Rab-1C;ras-related protein rab-1c (GTP-binding protein ray);ras-related protein Rab-35;RAY.
NCBI Gene Id11021
Protein NameNM_001167606, NP_001161078, Q15286
Description of TargetHomo sapiens RAB35, member RAS oncogene family (RAB35), transcript variant 2, mRNA, Ras-related protein Rab-35, ras-related protein Rab-35 isoform 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q15286
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001161078
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001167606
Molecular Weight17.5 kDa
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