Catalog No: OKEH08619
Size:96T
Price: $725.00
SKU
OKEH08619
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology RAB27B ELISA Kit (Human) (OKEH08619) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB27B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB27B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAB27B captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.8% (n = 20) Mean Inter-assay CV%: <=7.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: May be involved in targeting uroplakins to urothelial apical membranes. | ||||||||||||||||||||||
:: | Subcellular Location: Membrane Lipid-anchor | ||||||||||||||||||||||
:: | Unigene: Hs.25318 SMR: O00194 String: 9606.ENSP00000262094 | ||||||||||||||||||||||
:: | KEGG: hsa:5874 MIM: 603869 Pfam: PF00071 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.097ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Ras-related protein Rab-27B | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | RAB27B |
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Gene Full Name | RAB27B, member RAS oncogene family |
Alias Symbols | C25KG;ras-related protein Rab-27B. |
NCBI Gene Id | 5874 |
Protein Name | O00194 |
Description of Target | Ras-related protein Rab-27B |
Uniprot ID | https://www.uniprot.org/uniprot/O00194 |
Protein Accession # | NP_004154.2 |
Nucleotide Accession # | NM_004163.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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