RAB1A Chemi-Luminescent ELISA Kit (Human) (OKCD03893)

Catalog No: OKCD03893

Size:96WELLS

Human RAB1A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to RAB1A, Member RAS Oncogene Family (RAB1A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RAB1A, Member RAS Oncogene Family (RAB1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the RAB1A, Member RAS Oncogene Family (RAB1A) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB1A, Member RAS Oncogene Family (RAB1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB1A, Member RAS Oncogene Family (RAB1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • RAB1A Microplate: 96 Wells (12 x 8 Well strips)
  • RAB1A Lyophilized Standard: 2
  • 100X RAB1A Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RAB1A
• Gene Full Name: RAB1A, member RAS oncogene family
• Alias Symbols: GTP binding protein Rab1a;Rab GTPase YPT1 homolog;RAB1;RAB1, member RAS oncogene family;ras-related protein Rab-1A;YPT1;YPT1-related protein.
• NCBI Gene Id: 5861
• Protein Name: Ras-related protein Rab-1A
• Description of Target: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
• Uniprot ID: P62820
• Protein Accession #: NP_004152
• Nucleotide Accession #: NM_004161