RAB14 Antibody - C-terminal region (AVARP13107_P050)

Catalog No: AVARP13107_P050

Host: Rabbit
Target Name: RAB14
Sample Tissue: Human Fetal Liver
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: RAB14
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 1ug/ml

Lanes:
Murin JAWS-II cell line Primary Antibody Dilution:
1:100 Secondary Antibody:
Anti-rabbit-FITC Secondary Antibody Dilution:
1:1000 Gene Name:
Blue: Nucleus Green: Rab14 Submitted by:
RAB14

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Pig, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RAB14
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: FLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQREGCG
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-RAB14 (AVARP13107_P050) antibody is Catalog # AAP30756 (Previous Catalog # AAPP01417)
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that RAB14 is expressed in HepG2

• Reference: Junutula,J.R., (2004) Mol. Biol. Cell 15 (5), 2218-2229
• Publications:

  Farhoud, M. H. et al. Impaired ubiquitin-proteasome-mediated PGC-1a protein turnover and induced mitochondrial biogenesis secondary to complex-I deficiency. Proteomics 12, 1349-62 (2012). 22589185

  Kanzaki, H. et al. Identification of direct targets for the miR-17-92 cluster by proteomic analysis. Proteomics 11, 3531-9 (2011). 21751348

  Rab11-FIP1A regulates early trafficking into the recycling endosomes. Exp Cell Res. 340, 259-73 (2016). 26790954

  Rab14 regulation of claudin-2 trafficking modulates epithelial permeability and lumen morphogenesis. Mol Biol Cell. 25, 1744-54 (2014). 24694596

Target Info

• Gene Symbol: RAB14
• Gene Full Name: RAB14, member RAS oncogene family
• Alias Symbols: FBP, RAB-14
• NCBI Gene Id: 51552
• Protein Name: Ras-related protein Rab-14
• Description of Target: Localized to biosynthetic and endosomal compartments, Rab14 is involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.
• Uniprot ID: P61106
• Protein Accession #: NP_057406
• Nucleotide Accession #: NM_016322
• Protein Size (# AA): 215
• Molecular Weight: 24kDa
• Protein Interactions: EXOC5; UBC; RPA3; RPA2; RPA1; EED; RAB11FIP1; RAB11FIP5; OPTN; IQCB1; UBL4A; ATF2; VCP; ILVBL; TACC3; LAMTOR3; UQCRFS1; ILF3; HSPA5; HNRNPF; ABCC2; APP; RUFY1; RBSN; SMURF2; OCRL; CHM; DSTYK;