QSOX1 ELISA Kit (Mouse) (OKCD01206)

Catalog No: OKCD01206 (Formerly GWB-KBBHI6)

Size:96WELLS

Mouse Qsox1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-105%	80-102%	97-105%	80-92%
  EDTA plasma(n=5)	94-102%	83-97%	81-88%	80-89%
  heparin plasma(n=5)	90-103%	97-105%	94-103%	89-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	92-101	95
  EDTA plasma(n=5)	95-104	98
  heparin plasma(n=5)	97-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Qsox1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Qsox1 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Qsox1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 30 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). No significant cross-reactivity or interference between Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Qsox1
• Gene Full Name: quiescin Q6 sulfhydryl oxidase 1
• Alias Symbols: 1300003H02Rik;b2b2673C;b2b2673Clo;QS;Qsc;Qscn6;QSOX;Quiescin Q6;skin sulfhydryl oxidase;SOx;sulfhydryl oxidase 1.
• NCBI Gene Id: 104009
• Protein Name: Sulfhydryl oxidase 1
• Description of Target: Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins (By similarity).
• Uniprot ID: Q8BND5
• Protein Accession #: NP_001004128
• Nucleotide Accession #: NM_001004128.2
• Protein Size (# AA): 748