Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH08809
Size:96T
Price: $725.00
SKU
OKEH08809
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)84-94% (n = 20)94-103% (n = 20)101-112% (n = 20)86-96% (n = 20)
EDTA plasma(n=5)108-119% (n = 20)107-117% (n = 20)86-96% (n = 20)107-118% (n = 20)
heparin plasma(n=5)101-109% (n = 20)81-90% (n = 20)83-92% (n = 20)101-111% (n = 20)
ELISA Kit PrincipleAviva Systems Biology QSOX1 ELISA Kit (Human) (OKEH08809) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for QSOX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for QSOX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample QSOX1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.9% (n = 20)
Mean Inter-assay CV%: <=6.8% (n = 20)
ELISA Kit Component
ComponentAmount
QSOX1 Microplate96 Wells (12 x 8 Well strips)
QSOX1 Lyophilized Standard2
100X Biotinylated QSOX1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being involved in growth regulation.
::Subcellular Location: Isoform 2 Secreted Extracellular space Found in the extracellular medium of quiescent cells but is not found in proliferating cells.
::Unigene: Hs.744925
SMR: O00391
String: 9606.ENSP00000356574
::KEGG: hsa:5768
MIM: 603120
Pfam: PF04777
Reconstitution and StorageStore as indicated in product manual.
Sensitivity23pg/mL
SpecificityNatural and recombinant Human Sulfhydryl oxidase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolQSOX1
Gene Full Namequiescin sulfhydryl oxidase 1
Alias SymbolsQ6;QSCN6;Quiescin Q6;quiescin Q6 sulfhydryl oxidase 1;sulfhydryl oxidase 1;testis tissue sperm-binding protein Li 62n;thiol oxidase 1.
NCBI Gene Id5768
Protein NameO00391
Description of TargetSulfhydryl oxidase 1
Uniprot IDhttps://www.uniprot.org/uniprot/O00391
Protein Accession #NP_001004128.1
Nucleotide Accession #NM_001004128.2
Write Your Own Review
You're reviewing:QSOX1 ELISA Kit (Human) (OKEH08809)
Your Rating