Catalog No: OKEH08809
Size:96T
Price: $725.00
SKU
OKEH08809
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology QSOX1 ELISA Kit (Human) (OKEH08809) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for QSOX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for QSOX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample QSOX1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.9% (n = 20) Mean Inter-assay CV%: <=6.8% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being involved in growth regulation. | ||||||||||||||||||||||
:: | Subcellular Location: Isoform 2 Secreted Extracellular space Found in the extracellular medium of quiescent cells but is not found in proliferating cells. | ||||||||||||||||||||||
:: | Unigene: Hs.744925 SMR: O00391 String: 9606.ENSP00000356574 | ||||||||||||||||||||||
:: | KEGG: hsa:5768 MIM: 603120 Pfam: PF04777 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 23pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Sulfhydryl oxidase 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | QSOX1 |
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Gene Full Name | quiescin sulfhydryl oxidase 1 |
Alias Symbols | Q6;QSCN6;Quiescin Q6;quiescin Q6 sulfhydryl oxidase 1;sulfhydryl oxidase 1;testis tissue sperm-binding protein Li 62n;thiol oxidase 1. |
NCBI Gene Id | 5768 |
Protein Name | O00391 |
Description of Target | Sulfhydryl oxidase 1 |
Uniprot ID | https://www.uniprot.org/uniprot/O00391 |
Protein Accession # | NP_001004128.1 |
Nucleotide Accession # | NM_001004128.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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