QDPR ELISA Kit (Bovine) (OKEH08062)

Catalog No: OKEH08062

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology QDPR ELISA Kit (Bovine) (OKEH08062) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for QDPR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for QDPR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample QDPR captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.7% (n = 20)
  Mean Inter-assay CV%: <=8.1% (n = 20)
• ELISA Kit Component:
  • QDPR Microplate: 96 Wells (12 x 8 Well strips)
  • QDPR Lyophilized Standard: 2
  • 100X Biotinylated QDPR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases.
  Unigene: Bt.1735
  SMR: Q3T0Z7
  String: 9913.ENSBTAP00000049659
  KEGG: bta:618084
  Pfam: PF00106
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Dihydropteridine reductase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: QDPR
• Gene Full Name: quinoid dihydropteridine reductase
• Alias Symbols: dihydropteridine reductase;HDHPR;Quinoid dihydropteridine reductase.
• NCBI Gene Id: 618084
• Protein Name: Q3T0Z7
• Description of Target: Dihydropteridine reductase
• Uniprot ID: https://www.uniprot.org/uniprot/Q3T0Z7
• Protein Accession #: NP_001069960.1
• Nucleotide Accession #: NM_001076492.2