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Catalog No: OKCD02834
Size:96WELLS
Price: $750.00
SKU
OKCD02834
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Datasheets/ManualsPrintable datasheet for PYY ELISA Kit (Pig) (OKCD02834)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)79-93%82-96%91-101%80-99%
EDTA Plasma (n=5)78-91%79-102%78-88%94-101%
Heparin Plasma (n=5)85-99%86-95%94-102%96-104%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Peptide YY (PYY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Peptide YY (PYY) and unlabeled Peptide YY (PYY) (Standards or samples) with the pre-coated antibody specific to Peptide YY (PYY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Peptide YY (PYY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Peptide YY (PYY) in the sample.
ELISA Kit Range12.35-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-9989
EDTA Plasma (n=5)80-9082
Heparin Plasma (n=5)94-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptide YY (PYY) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptide YY (PYY) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PYY Microplate96 Wells (12 x 8 Well strips)
PYY Lyophilized Standard 2 x 1 ng
100X PYY Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 4.51 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptide YY (PYY). No significant cross-reactivity or interference between Peptide YY (PYY) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Inhibition ELISA
Gene SymbolPYY
Gene Full NamePeptide YY
Alias SymbolsPeptide tyrosine tyrosine.
NCBI Gene Id445018
Protein NamePeptide YY
Description of TargetThis gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
Uniprot IDP68005
Molecular Weight36 kDa
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