PYY ELISA Kit (Human) : 96 Wells (OKEH00737)

Catalog No: OKEH00737

Size:96T

Human PYY ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PYY ELISA Kit (Human) (OKEH00737) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PYY antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PYY and incubated. The PYY found in the sample or standards competes with the biotinylated PYY for limited binding sites on the immobilized anti-human PYY antibody. Excess unbound biotinylated PYY and sample or standard PYY is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PYY captured in the well and inversely proportional to the amount of PYY which was contained in the sample or standard.
• ELISA Kit Range: 6.25-400pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.1% (n = 20)
  Mean Inter-assay CV%: < 6.9% (n = 20)
• ELISA Kit Component:
  • Anti-PYY Microplate: 96 Wells (12 x 8 Well strips)
  • PYY Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X PYY-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 3.1 pg/mL
• Specificity: Natural and recombinant Human Peptide YY
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PYY
• Gene Full Name: peptide YY
• Alias Symbols: peptide tyrosine tyrosine;peptide YY;prepro-PYY;PYY1;PYY-I.
• NCBI Gene Id: 5697
• Protein Name: P10082
• Description of Target: Peptide YY
• Uniprot ID: https://www.uniprot.org/uniprot/P10082
• Protein Accession #: NP_004151.3
• Nucleotide Accession #: NM_004160.5
• Protein Size (# AA): 97
• Molecular Weight: 11 kDa