PKM2 Antibody - C-terminal region (OAAB16784)

Catalog No: OAAB16784

Size:200ul

Western blot analysis of anti-h PKM2-N491 Pab (Cat. #OAAB16784) in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab.

Pyruvate Kinase (PKM2) Antibody (C-term) (Cat. #OAAB16784) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB16784 Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Precautions: Pyruvate Kinase (PKM2) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival
  Cellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity
  Tissue Location: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:2000
  WB: 1:1000
  IF: 1:200
  IHC-P: 1:50~1:100

Target Info

• Gene Symbol: PKM
• Alias Symbols: PK3, TCB, p58, OIP3, PKM2, CTHBP, THBP1, HEL-S-30
• NCBI Gene Id: 5315
• Protein Name: Pyruvate kinase isozymes M1/M2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P14618
• Protein Accession #: NP_001193725.1
• Molecular Weight: 57937 Da