Catalog No: OKCD06900
Size:96WELLS
Price: $650.00
SKU
OKCD06900
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Datasheets/ManualsPrintable datasheet for PYGL ELISA Kit (Rat) (OKCD06900)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Phosphorylase, Liver (PYGL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Phosphorylase, Liver (PYGL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Phosphorylase, Liver (PYGL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycogen Phosphorylase, Liver (PYGL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycogen Phosphorylase, Liver (PYGL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycogen Phosphorylase, Liver (PYGL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PYGL Microplate96 Wells (12 x 8 Well strips)
PYGL Lyophilized Standard2
100X PYGL HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.56ng/mL
Gene SymbolPygl
Gene Full Nameglycogen phosphorylase L
Alias Symbolsglycogen phosphorylase, liver form;liver glycogen phosphorylase;phosphorylase, glycogen, liver.
NCBI Gene Id64035
Protein NameGlycogen phosphorylase, liver form
Description of TargetPhosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Uniprot IDP09811
Protein Accession #NP_071604.1
Nucleotide Accession #NM_022268.1
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