PYCARD Antibody - N-terminal region (OASG00582)

Catalog No: OASG00582

Size:100 uL

Western Blot using 823, 293T

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, IF
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from the N-terminal region of human ASC. Location: 10-90aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: ASC Polyclonal Antibody detects endogenous levels of ASC protein.
• Application Info: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Target Info

• Gene Symbol: PYCARD
• Gene Full Name: PYD and CARD domain containing
• Alias Symbols: ASC, TMS, TMS1, CARD5, TMS-1
• NCBI Gene Id: 29108; 66824;
• Description of Target: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q9ULZ3, Q9EPB4
• Molecular Weight: 21 kDa