Pxd Antibody : Biotin (OASE00137-Biotin)

Catalog No: OASE00137-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Species Independent
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: N1664A
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: Immuno-spin Trapping, ELISA, WB, ICC, IF, IP
• Additional Information: Background Info: Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
  Scientific Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis
  of many disease states (1). The ability to identify these
  species is crucial, and spin trapping has accomplished
  this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of
  the least toxic to cells and animals, and possesses convenient
  pharmacokinetics (uptake, distribution, metabolism and
  excretion) in biological systems (2-6).
  Recent studies have determined that nitric oxide may
  substantially affect the quantitative determination of DMPO
  adducts, and therefore extra caution is required when studying
  generation of these species in the presence of nitric oxide or
  its radicals (1). DMPO adducts can be generated with protein
  and DNA radicals (7).
  Certificate of Analysis: A 1/1000 dilution was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1-10ug/mL (ELISA),10ug/mL (ICC), 25ug (IP)
• Storage Buffer: TBS pH7.4, 0.05% sodium azide, 50% glycerol
• Reference: 1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007)
  Nat Protoc. 2(3): 512-522.
  3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473–
  1481.
  4. Haseloff R.F., et al. (1997) FEBS Lett 418:73–75.
  5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and
  Kosanke S.D. (1996) Free Radic. Biol. Med 21:427–436.
  6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251–
  256.
  7. Free Radic Biol Med. 2009 April 1; 46(7): 853–865.
  doi:10.1016/j.freeradbiomed.2008.12.020.
  8. Chatterjee S., Ehrenshaft, M., Bhattacharjee ,S.,
  Derterding, L.J., Bonini, M.G., Corbett, J., Kadiiska,
  M.B., Tomer K.B. and Mason, R.P. 2009 Free Radic. Med.
  and Biol. 46:454-461.

Target Info

• Gene Symbol: Pxd
• Gene Full Name: Peroxidase
• Alias Symbols: CG3477, Dmel\CG3477, DmPO, Dpxd, PO
• NCBI Gene Id: 2768671
• Protein Name: Peroxidase
• Uniprot ID: Q01603
• Protein Accession #: NP_001163633.1; NP_996223.1
• Nucleotide Accession #: NM_001170162.2; NM_206501.3