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Catalog No: OKCD00114 (Formerly GWB-KBBOG7)
Size:96WELLS
Price: $650.00
SKU
OKCD00114
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Datasheets/ManualsPrintable datasheet for PVR ELISA Kit (Human) (OKCD00114)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)90-101%84-102%98-105%95-104%
EDTA plasma(n=5)79-93%78-95%85-101%89-96%
heparin plasma(n=5)79-92%78-93%97-105%86-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor (PVR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poliovirus Receptor (PVR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poliovirus Receptor (PVR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Poliovirus Receptor (PVR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)84-9489
EDTA plasma(n=5)86-9793
heparin plasma(n=5)78-9692
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poliovirus Receptor (PVR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poliovirus Receptor (PVR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PVR Microplate96 Wells (12 x 8 Well Strips)
PVR Lyophilized Standard2 x 1 ng
100X Biotinylated PVR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.0 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Poliovirus Receptor (PVR). No significant cross-reactivity or interference between Poliovirus Receptor (PVR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPVR
Gene Full NamePVR cell adhesion molecule
Alias SymbolsCD155;HVED;Necl-5;NECL5;nectin-like protein 5;poliovirus receptor;PVS;TAGE4.
NCBI Gene Id5817
Protein NamePoliovirus receptor
Description of TargetMediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.
Uniprot IDP15151
Protein Accession #NP_001129240
Nucleotide Accession #NM_001135768.2
Protein Size (# AA)417
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