Size:100ug
$350.00
Discontinued
SKU
OASE00386
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Conjugation Options

OASE00386-FITC Conjugated

OASE00386-HRP Conjugated

OASE00386-Biotin Conjugated

PUMA (NT) Antibody (OASE00386)

Catalog#: OASE00386
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This product is Genway GWB-0X0025
More Information
Predicted Species Reactivity Human
Clonality Polyclonal
Host Rabbit
Application WB, IHC
Additional Information Background Info: Detects a ~23kDa protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kDa bands may be seen in some instances, possibly corresponding to PUMAbeta.
:: Scientific Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
:: Certificate of Analysis: 2 ug/mL was sufficient for detection of PUMA in 20ug of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Reconstitution and Storage Store at -20C. Shipping in Blue Ice or 4C.
Immunogen N-terminal amino acids of human PUMA
Purification Affinity Purified
Concentration 1mg/mL
Datasheets/Manuals Printable datasheet for OASE00386
Dilution 1:500 (WB), 1:100 (ICC)
Target Reference 1. Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7(3):683-94.
2. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001;7(3):673-82.
3. Han J, Flemington C, Houghton AB , Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98(20):11318-23.
Storage Buffer PBS with 0.02% sodium azide
Gene Symbol TNG1
Official Gene Full Name TCL1-neighboring gene 1
NCBI Gene Id 27114
Swissprot Id Q9BXH2
Protein Accession # NP_001120712.1
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express TNG1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express TNG1.
  1. What is the species homology for "PUMA (NT) Antibody (OASE00386)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "PUMA (NT) Antibody (OASE00386)"?

    This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".

  3. What buffer format is "PUMA (NT) Antibody (OASE00386)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "PUMA (NT) Antibody (OASE00386)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "PUMA (NT) Antibody (OASE00386)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "PUMA (NT) Antibody (OASE00386)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "PUMA (NT) Antibody (OASE00386)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "PUMA (NT) Antibody (OASE00386)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "TNG1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "TNG1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "TNG1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "TNG1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "TNG1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "TNG1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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