Puma BH3 Domain Antibody (OAAB09482)

Catalog No: OAAB09482

Size:400ul

Western blot analysis of anti-Puma BH3 domain Pab (Cat. #OAAB09482) in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

BBC3 is strongly supported by BioGPS gene expression data to be expressed in HL60

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody (Cat.#OAAB09482), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Puma BH3 Domain Antibody (Cat. #OAAB09482) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of Puma BH3 Domain Antibody(Cat#OAAB09482) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF, FC
• Additional Information: Function: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.
  Cellular Location: Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release
  Tissue Location: Ubiquitously expressed.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  BBC3 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Intended Use: Puma BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: BBC3
• Alias Symbols: JFY1, PUMA, JFY-1
• NCBI Gene Id: 27113
• Protein Name: Bcl-2-binding component 3
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9BXH1
• Protein Accession #: NP_001120712.1
• Molecular Weight: 20532 Da