PUF60 Recombinant Protein (OPCD00128)

Catalog No: OPCD00128

PUF60 Recombinant Protein (OPCD00128) using SDS-PAGE

PUF60 Recombinant Protein (OPCD00128) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Asn226-Gln549
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 90%
• Source: E.coli
• Tag: N-terminal His and GST Tag

Target Info

• Gene Symbol: PUF60
• Gene Full Name: poly(U) binding splicing factor 60
• Alias Symbols: 60 kDa poly(U)-binding-splicing factor;FBP interacting repressor;FIR;FUSE-binding protein-interacting repressor;poly(U) binding splicing factor 60KDa;poly(U)-binding-splicing factor PUF60;pyrimidine tract binding splicing factor;Ro ribonucleoprotein-binding protein 1;Ro-binding protein 1;RoBPI;Siah binding protein 1;Siah-binding protein 1;SIAHBP1;VRJS.
• NCBI Gene Id: 22827
• Protein Name: Poly(U)-binding-splicing factor PUF60
• Description of Target: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
• Uniprot ID: Q9UHX1
• Protein Accession #: NP_001129505.1
• Nucleotide Accession #: NM_001136033.2
• Protein Size (# AA): Asn226~Gln549
• Molecular Weight: 65kDa