PTTG1IP ELISA Kit (Human) : 96 Wells (OKEH00492)

Catalog No: OKEH00492

Size:96T

Human PTTG1IP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PTTG1IP ELISA Kit (Human) (OKEH00492) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human PTTG1IP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human PTTG1IP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human PTTG1IP captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.2% (n = 20)
  Mean Inter-assay CV%: < 7.7% (n = 20)
• ELISA Kit Component:
  • Anti-PTTG1IP Microplate: 96 Wells (12 x 8 Well strips)
  • PTTG1IP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated PTTG1IP: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Pituitary tumor-transforming gene 1 protein-interacting protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PTTG1IP
• Gene Full Name: PTTG1 interacting protein
• Alias Symbols: C21orf1;C21orf3;PBF;pituitary tumor-transforming 1 interacting protein;pituitary tumor-transforming gene 1 protein-interacting protein;pituitary tumor-transforming gene protein-binding factor;PTTG-binding factor.
• NCBI Gene Id: 754
• Protein Name: P53801
• Description of Target: Pituitary tumor-transforming gene 1 protein-interacting protein
• Uniprot ID: https://www.uniprot.org/uniprot/P53801
• Protein Accession #: NP_001273751.1
• Nucleotide Accession #: NM_001286822.1
• Protein Size (# AA): 180
• Molecular Weight: 20 kDa