PTPRQ ELISA Kit (Human) : 96 Wells (OKEH02080)

Catalog No: OKEH02080

Size:96T

Human PTPRQ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PTPRQ ELISA Kit (Human) (OKEH02080) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human PTPRQ antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PTPRQ and incubated. The PTPRQ found in the sample or standards competes with the biotinylated PTPRQ for limited binding sites on the immobilized anti-Human PTPRQ antibody. Excess unbound biotinylated PTPRQ and sample or standard PTPRQ is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PTPRQ captured in the well and inversely proportional to the amount of PTPRQ which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.5% (n = 20)
  Mean Inter-assay CV%: < 8.7% (n = 20)
• ELISA Kit Component:
  • Anti-PTPRQ Microplate: 96 Wells (12 x 8 Well strips)
  • PTPRQ Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X HTR1B-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Human Phosphatidylinositol phosphatase PTPRQ
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: PTPRQ
• Gene Full Name: protein tyrosine phosphatase, receptor type Q
• Alias Symbols: Receptor-type tyrosine-protein phosphatase Q.
• NCBI Gene Id: 374462
• Protein Name: Q9UMZ3
• Description of Target: Phosphatidylinositol phosphatase PTPRQ
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UMZ3
• Protein Accession #: NP_001138498.1
• Nucleotide Accession #: NM_001145026.1
• Protein Size (# AA): 2299
• Molecular Weight: 257 kDa