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Catalog No: OKEI00530
Size:96 Wells
Price: $625.00
SKU
OKEI00530
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Ptprn ELISA Kit (Mouse) (OKEI00530) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptprn has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ptprn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Ptprn
ELISA Kit Range0.781 - 50 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Ptprn. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Ptprn using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
LACTB2 Microplate96 Wells (12 x 8 Well strips)
LACTB2 Lyophilized Standard2
100X LACTB2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity0.469 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPTPRN
Alias SymbolsIA-2, KIAA4064, mIA-A, mKIAA4064, Ptp35, PTP IA-2, Receptor-type tyrosine-protein phosphatase-like N, R-PTP-N
Protein NameReceptor-type tyrosine-protein phosphatase-like N
Description of TargetPlays a role in vesicle-mediated secretory processes . Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation . Plays a role in insulin secretion in response to glucose stimuli . Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain . In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) . Seems to lack intrinsic enzyme activity . Required to maintain normal levels of renin expression and renin release . May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization .
Uniprot IDQ60673
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