Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01983
Size:96T
Price: $600.00
SKU
OKEH01983
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

PTPRF ELISA Kit (Human) : 96 Wells (OKEH01983)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PTPRF ELISA Kit (Human) (OKEH01983) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTPRF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTPRF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PTPRF captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
PTPRF Microplate96 Wells (12 x 8 Well strips)
PTPRF Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated VASP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.43 ng/mL
SpecificityNatural and recombinant Human Receptor-type tyrosine-protein phosphatase F
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPTPRF
Gene Full Nameprotein tyrosine phosphatase receptor type F
Alias SymbolsBNAH2;LAR;LCA-homolog;leukocyte antigen-related (LAR) PTP receptor;leukocyte antigen-related tyrosine phosphatase;leukocyte common antigen related;protein tyrosine phosphatase, receptor type, F polypeptide;receptor-linked protein-tyrosine phosphatase LAR;receptor-type tyrosine-protein phosphatase F.
NCBI Gene Id5792
Protein NameP10586
Description of TargetReceptor-type tyrosine-protein phosphatase F
Uniprot IDhttps://www.uniprot.org/uniprot/P10586
Protein Accession #NP_002831.2
Nucleotide Accession #NM_002840.3
Protein Size (# AA)1907
Molecular Weight213 kDa
Write Your Own Review
You're reviewing:PTPRF ELISA Kit (Human) : 96 Wells (OKEH01983)
Your Rating
We found other products you might like!