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Ptprc Antibody (OASA05279)

Catalog#: OASA05279
This product is Genway GWB-B97C8D
More Information
Predicted Species Reactivity Mouse
Product Format Purified IgG - liquid
Clonality Monoclonal
Clone IBL-3/16
Isotype IgG1
Host Rat
Application IHC-AFF, FC, IP
Additional Information Fusion Partners: Spleen cells from an immunised Wistar rat were fused with cells of the SP2/0-Ag14 mouse myeloma cell line
:: Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:: Approx Protein Conc: IgG concentration 0.5 mg/ml
Buffer Solutions: TRIS buffered saline
Reconstitution and Storage Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Replacement Item This antibody may replace item sc-1123 from Santa Cruz Biotechnology.
Immunogen The immunogen for anti-Ptprc antibody: purified B cells from mouse lymph nodes
Predicted Homology Based on Immunogen Sequence Mouse
Datasheets/Manuals Printable datasheet for anti-Ptprc antibody - OASA05279
Specificity CD45
Application Info Flow Cytometry:    1/50 - 1/100
Immunohistology - Frozen:    1/50 - 1/100
Protocol Information Citation: 1: Copland DA, Calder CJ, Raveney BJ, Nicholson LB, Phillips J, Cherwinski H, Jenmalm M, Sedgwick JD, Dick AD. Monoclonal antibody-mediated CD200 receptor signaling suppresses macrophage activation and tissue damage in experimental autoimmune uveoretinitis. Am J Pathol. 2007 Aug;171(2):580-8. Epub 2007 Jun 28. PubMed PMID: 17600119; PubMed Central PMCID: PMC1934542.
Species: Mouse
Experiment Name: 1. EAU Induction and Scoring2. Isolation of Retinal Myeloid Cells
Experiment Background: 1. In this study, David et al. show that inducing myeloid CD200R signaling via the systemic administration of an agonist rat anti-mouse mAb, DX109 successfully suppresses an archetypal organ-specific T-cell-mediated disease, EAU.2. A model of CD4+ T-cell organ-specific autoimmunity, experimental autoimmune uveoretinitis (EAU) was used in these studies because of extensive neuronal CD200 expression in the eye where tissue destruction is largely mediated via activated macrophages and NO damage. Systemic administration of a rat anti-mouse CD200R monoclonal antibody (mAb) (DX109) resulted in suppression of EAU and tissue damage even in the presence of systemic T-cell proliferation and IFNg production.
Experimental Steps: 1. EAU Induction and Scoring: C57BL/6 and CD200-/- mice were immunized subcutaneously in one flank with 500 ug/mouse hRBP-3 1-20 peptide in phosphate (PBS) (2% dimethyl sulfoxide), in emulsion with complete Freud’s adjuvant [1 mg/ml; 1:1 (v/v)] supplemented with 1.5 mg/ml Mycobacterium tuberculosis complete H37 RA (BD Biosciences) and 1.5 ug of Bordetella pertussis toxin (Sigma-Aldrich, Dorset, UK) intraperitoneally (i.p.). B10.RIII mice were immunized subcutaneously in one flank with 50 ug/mouse IRBP 161-180 peptide in PBS (2% dimethyl sulfoxide), in complete Freud’s adjuvant, with an additional i.p. injection of 1 ug of Bordetella pertussis toxin. At various time points after immunization, eyes were enucleated and carefully snap-frozen, oriented in optimal cutting temperature compound (R. Lamb Ltd., East Sussex, UK). Serial 12-um sections were thawed at room temperature and fixed in acetone for 10 minutes. Sections were stained with rat anti-mouse monoclonal anti-CD45 antibody (Oxford, UK) and counterstained with hematoxylin (ThermoShandon, Pittsburgh, PA). Sections were scored for inflammatory infiltrate (presence of CD45-positive cells) and structural disease (disruption of morphology).2. Isolation of Retinal Myeloid Cells: Eyes were enucleated from immunized mice, and the retinas of each animal were dissected microscopically in ice-cold 1% bovine serum albumin (Sigma-Aldrich) in PBS (1% PBSA). Retinas were then mechanically disrupted through a 40-um cell strainer to obtain a singlecell suspension. A sample was removed to determine the purity and percentage of F4/80-positive macrophages by staining cells with fluorescein isothiocyanate-conjugated anti-mouse F4/80 before flow cytometric analysis. Cells were plated at 5 x 105 F4/80-positive cells/ml for 2 hours, to allow the isolation of macrophages from other cell contaminants via plastic adherence. The medium was replaced and assays performed.
Other Reagents Used: Penicillin, streptomycin, L-glutamine, sodium pyruvate, and 2-mercaptoethanol
Number Of Protocols: 2
Target Reference 1. Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different
subsets of microglia and macrophages after ischemic stroke in mice. J. Neuroinflammation. 5:46.
2. Balogh, P., Bal¡zs, M. and A. Kum¡novicx (1995) Modulatory effect of CD45 on the MHC Class II-induced homotypic aggregation of B cells in mice. In 9th International Congress of Immunology, Abstract book p. 72. No. 422.
Gene Symbol Ptprc
Alias Symbols loc, B220, Cd45, Ly-5, T200, CD45R, Lyt-4, Ptprc
NCBI Gene Id 19264
Protein Name Receptor-type tyrosine-protein phosphatase C
Description of Target RAT ANTI MOUSE CD45
Swissprot Id P06800
Protein Size (# AA) 1291
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Ptprc.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Ptprc.
  1. What is the species homology for "Ptprc Antibody (OASA05279)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Ptprc Antibody (OASA05279)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Ptprc Antibody (OASA05279)" provided in?

    This item is provided in "Purified IgG - liquid".
    Additional format options may be available. For more information please contact

  4. What are other names for "Ptprc Antibody (OASA05279)"?

    This target may also be called "loc, B220, Cd45, Ly-5, T200, CD45R, Lyt-4, Ptprc" in publications.

  5. What is the shipping cost for "Ptprc Antibody (OASA05279)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Ptprc Antibody (OASA05279)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Ptprc Antibody (OASA05279)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Ptprc Antibody (OASA05279)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "PTPRC"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "PTPRC"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "PTPRC"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "PTPRC"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "PTPRC"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "PTPRC"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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