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Ptprc Antibody (OASA05278)

25 ug
$129.00
In Stock
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Gene Symbol:
Ptprc
NCBI Gene Id:
19264
Protein Name:
Receptor-type tyrosine-protein phosphatase C
Swissprot Id:
P06800
Alias Symbols:
loc, B220, Cd45, Ly-5, T200, CD45R, Lyt-4, Ptprc
Replacement Item:
This antibody may replace item sc-1123 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI MOUSE CD45
Protein Size (# AA):
1291
Host:
Rat
Clonality:
Monoclonal
Application:
IHC-AFF, FC, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Ptprc.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Ptprc.
Immunogen:
The immunogen for anti-Ptprc antibody: mouse spleen cells.
Predicted Species Reactivity:
Mouse
Predicted Homology Based on Immunogen Sequence:
Mouse
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Ptprc antibody - OASA05278
Specificity:
CD45
Clone:
YW62.3
Isotype:
IgG2b
Application Info:
Flow Cytometry:    1/100 - 1/200
Application Data:
Application #1: Staining of mouse splenocytes with Rat anti Mouse CD45: FITC
Application #2: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD45 (Ly-5):Alexa Fluor 647
Application #3: Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5):RPE
Application #4: Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5)
Application #5: Staining of mouse spleen with Rat anti Mouse CD45
Additional Information:
Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Watt, S.M. et al. (1987) Cell surface markers on haemapoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations. Mol. Cell. Probes 1: 297-326.
2. Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. Eur. J. Immunol. 18: 1507-1514.

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