- NCBI Gene Id:
- Protein Name:
- Receptor-type tyrosine-protein phosphatase C
- Alias Symbols:
- loc, B220, Cd45, Ly-5, T200, CD45R, Lyt-4, Ptprc
- Replacement Item:
- This antibody may replace item sc-1123 from Santa Cruz Biotechnology.
- Description of Target:
- RAT ANTI MOUSE CD45
- Protein Size (# AA):
- IHC-AFF, FC, IP
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express Ptprc.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express Ptprc.
- The immunogen for anti-Ptprc antibody: mouse spleen cells.
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-Ptprc antibody - OASA05278
- Application Info:
- Flow Cytometry: 1/100 - 1/200
- Application Data:
- Application #1: Staining of mouse splenocytes with Rat anti Mouse CD45: FITC
Application #2: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD45 (Ly-5):Alexa Fluor 647
Application #3: Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5):RPE
Application #4: Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5)
Application #5: Staining of mouse spleen with Rat anti Mouse CD45
- Additional Information:
- Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
- Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
- Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
- Target Reference:
- 1. Watt, S.M. et al. (1987) Cell surface markers on haemapoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations. Mol. Cell. Probes 1: 297-326.
2. Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. Eur. J. Immunol. 18: 1507-1514.