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Ptprc Antibody (OASA04004)

1 mg
$759.00
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Gene Symbol:
Ptprc
NCBI Gene Id:
24699
Protein Name:
Receptor-type tyrosine-protein phosphatase C
Swissprot Id:
P04157
Protein Accession #:
NP_001103357.1
Alias Symbols:
Lca, RT7, CD45, L-CA, T200, Ptprc
Replacement Item:
This antibody may replace item sc-1123 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI RAT CD45RA (B CELLS ONLY)
Protein Size (# AA):
1190
Host:
Mouse
Clonality:
Monoclonal
Application:
IHC-AFF, FC, IHC-FFPE
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Ptprc.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Ptprc.
Immunogen:
The immunogen for anti-Ptprc antibody: purified spleen leucocyte common antigen
Predicted Species Reactivity:
Rat
Predicted Homology Based on Immunogen Sequence:
Rat
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Ptprc antibody - OASA04004
Specificity:
CD45RA
Clone:
OX-33
Isotype:
IgG1
Application Info:
Flow Cytometry:    1/50 - 1/100
>
(1) This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) - refer to ref. Whiteland, J.L. et al. (1995) - reference 4.
Application Data:
Application #1: Staining of rat spleen cells with Mouse anti Rat CD45RA: FITC
Application #2: Staining of rat spleen cells with Mouse anti Rat CD45RA:Alexa Fluor 488
Additional Information:
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
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Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.2
Target Reference:
1. Woollett, G. R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur. J. Immunol. 15:168-173.
2. Barclay, A.N. et al. (1987) Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus. EMBO J. 6:1259-1264.
3. Barclay, A.N. et al. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 42:593-600.
4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leucocytes in Paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.

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