Ptprc Antibody (OASA03993)

Catalog#: OASA03993

• Size: 0.1 mg

• Predicted Species Reactivity: Rat
• Product Format: Purified IgG - liquid
• Clonality: Monoclonal
• Clone: OX-1
• Isotype: IgG1
• Host: Mouse
• Application: IHC-AFF, FC
• Additional Information: Fusion Partners: Spleen cells from BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
  Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 1.0 mg/ml
• Reconstitution and Storage: Store at +4^oC or at -20^oC if preferred.
  This product should be stored undiluted.
  Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
  
• Gene Symbol: Ptprc
• NCBI Gene Id: 24699
• Protein Name: Receptor-type tyrosine-protein phosphatase C
• Swissprot Id: P04157
• Protein Accession #: NP_001103357.1
• Alias Symbols: Lca, RT7, CD45, L-CA, T200, Ptprc
• Replacement Item: This antibody may replace item sc-1123 from Santa Cruz Biotechnology.
• Description of Target: MOUSE ANTI RAT CD45
  
• Protein Size (# AA): 1190
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express Ptprc.
• Immunogen: The immunogen for anti-Ptprc antibody: rat thymocyte membrane glycoproteins
• Predicted Homology Based on Immunogen Sequence: Rat
• Specificity: CD45
• Application Info: Flow Cytometry:    1/100
  
• Application Data: Application #1: Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD45
  Application #2: Staining of rat spleen cells with Mouse anti Rat CD45: RPE
  Application #3: Staining of rat spleen cells with Mouse anti Rat CD45:Alexa Fluor 488
  Application #4: Staining of rat spleen cells with Mouse anti Rat CD45:FITC
• Target Reference: 1. Sunderland, C. A. et al. (1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes. Eur. J. Immunol. 9:155-159
  2. Woollett, G. R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B Lymphocytes. Eur. J. Immunol. 15:168-173
  3. Martin, A. et al. (1995) Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis. Clin. Exp. Immunol. 99:283-288
  4. Sato, K et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants. J. Immunol. 166:4185-4194
  5. Murakami, K. et al. (2000) Regulation of mast cell signalling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase. Int. Immunol. 12(2):169-176
  6. Standring, R. et al. (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens. Eur. J. Immunol. 8(12):832-9.
  7. Giezeman-Smits, K.M. et al. (1999) The regulatory role of CD45 on rat NK cells in target cell lysis. J. Immunol. 163(1):71-6.
  8. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and

Protocol:

Tips Information: