- NCBI Gene Id:
- Protein Name:
- Receptor-type tyrosine-protein phosphatase C
- Alias Symbols:
- LCA, LY5, B220, CD45, T200, CD45R, GP180, PTPRC
- Replacement Item:
- This antibody may replace item sc-1123 from Santa Cruz Biotechnology.
- Description of Target:
- MOUSE ANTI HUMAN CD45RO
- Protein Size (# AA):
- IHC-AFF, FC, IP, IHC-FFPE
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express PTPRC.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express PTPRC.
- The immunogen for anti-PTPRC antibody: human IL-2 dependent T cells
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-PTPRC antibody - OASA02097
- Application Info:
- Flow Cytometry: 1/25 - 1/50
- Application Data:
- Application #1: Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO
Application #2: Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO
Application #3: Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO:Azide Free
- Additional Information:
- Fusion Partners: Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.
Histology Positive Control Tissue: Tonsil
- Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
- Approx Protein Conc: IgG concentration 1 mg/ml
Buffer Solutions: Phosphate buffered saline
- Target Reference:
- 1. Smith, S.H. et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology 58: 63-70.
2. Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T-cells in routinely fixed tissues. J. Clin. Pathol. 39: 399-405.
3. Beverley, P.C.L. et al. (1986) T-cell subsets and function. Progress in Immunology VI. Cinader, B., Miller, G.G., eds. Academic Press Orlando pp 941-948.
4. Beverley, P.C.L. (1987) Human Tcell subsets. Immunology Letters 14: 263-267.
5. Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets. Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-227.
6. Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T-cells. J. Immunol. 140: 2171 - 2178
7. Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45 Immunology 64: 331-336.
8. Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function. Immunology, Suppl. 1: 3-5.
9. Merkenschlager, M. et al. (1988) Limited dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T-cell memory formation. Eur. J. Immunol. 18: 1653-1661.