PTPRC Antibody (OAEE00253)

Catalog No: OAEE00253

Size:100UG

Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD45R0 antibody UCHL1. 

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: UCHL1
• Isotype: IgG2a
• Host: Mouse
• Application: Flow cytometry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Human IL-2 dependent T cells
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody UCHL1 recognizes CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.
  HLDA III; WS Code NL 826
  HLDA III; WS Code T 128
  HLDA IV; WS Code NL 31
  HLDA V; WS Code BP BP460
  HLDA V; WS Code T T-081
  HLDA V; WS Code T T-CD45.43
  
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG: Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986 Apr;39(4):399-405.
  Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC: Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986 May;58(1):63-70.
  Akbar AN, Terry L, Timms A, Beverley PC, Janossy G: Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1;140(7):2171-8.
  Terry LA, Brown MH, Beverley PC: The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the humanleucocyte-common antigen, CD45. Immunology. 1988 Jun;64(2):331-6.
  Beverley PC, Merkenschlager M, Terry L: Phenotypic diversity of the CD45 antigen and its relationship to function. Immunol Suppl. 1988;1:3-5.
  Merkenschlager M, Terry L, Edwards R, Beverley PC: Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur J Immunol. 1988 Nov;18(11):1653-61.
  And many other.
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Beverley PC.: Human T cell subsets. Immunol Lett. 1987 Apr;14(4):263-7.
  Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19
  Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90.
  Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25.
  Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65.
  Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302.

Target Info

• Gene Symbol: PTPRC
• Gene Full Name: Receptor-type tyrosine-protein phosphatase C
• Alias Symbols: LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180
• NCBI Gene Id: 5788
• Protein Name: Receptor-type tyrosine-protein phosphatase C
• Uniprot ID: P08575
• Protein Accession #: NP_002829.3; NP_563578.2
• Nucleotide Accession #: NM_002838.4; NM_080921.3