PTPRC Antibody (OAEE00251)

Catalog No: OAEE00251

Size:100UG

Product Info

• Predicted Species Reactivity: Cat|Human|Mouse
• Product Format: Phosphate buffered saline (PBS), pH 7.4
• Clonality: Monoclonal
• Clone: RA3-6B2
• Isotype: IgG2a
• Host: Rat
• Application: Flow cytometry|Functional assay|Immunocytochemistry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The rat monoclonal antibody RA3-6B2 recognizes an epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
• Formulation: Low endotoxin
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Reference: Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004 Apr 1;172(7):4298-307.
  Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B-1 B cell-specified progenitor. Nat Immunol. 2006 Mar;7(3):293-301.
  Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A. 2006 Apr;69(4):249-59.
  Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 1998 Jul;9(1):135-41.
  Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem. 2000 May;48(5):653-62.
  Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. Scand J Immunol. 1994 May;39(5):419-25.
  

Target Info

• Gene Symbol: PTPRC
• Gene Full Name: Receptor-type tyrosine-protein phosphatase C
• Alias Symbols: LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180
• NCBI Gene Id: 5788
• Protein Name: Receptor-type tyrosine-protein phosphatase C
• Uniprot ID: P08575
• Protein Accession #: NP_002829.3; NP_563578.2
• Nucleotide Accession #: NM_002838.4; NM_080921.3