PTPRC Antibody (OAEE00246)

Catalog No: OAEE00246

Size:100UG

Surface staining of human peripheral blood cells with anti-human CD45 (HI30) purified, GAM-APC.

Product Info

• Predicted Species Reactivity: Human|Non-human Primate
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: HI30
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunocytochemistry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: human peripheral blood leukocytes
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The mouse monoclonal antibody HI30 recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
  HLDA IV; WS Code N816
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Tembhare PR, Subramanian PG, Sehgal K, Yajamanam B, Kumar A, Gadge V, Inamdar N, Gujral S: Immunophenotypic profile of plasma cell leukemia: a retrospective study in areference cancer center in India and review of literature. Indian J Pathol Microbiol. 2011 Apr-Jun;54(2):294-8.
  Cubillos-Ruiz JR, Martinez D, Scarlett UK, Rutkowski MR, Nesbeth YC, Camposeco-Jacobs AL, Conejo-Garcia JR: CD277 is a negative co-stimulatory molecule universally expressed by ovarian cancer microenvironmental cells. Oncotarget. 2010 Sep;1(5):329-38.
  Song L, Ara T, Wu HW, Woo CW, Reynolds CP, Seeger RC, DeClerck YA, Thiele CJ, Sposto R, Metelitsa LS: Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma. J Clin Invest. 2007 Sep;117(9):2702-12.
  Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow-derived stem cells after myocardial infarction andleft ventricular function. Eur Heart J. 2005 Jun;26(12):1196-204.
  Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW Jr, Arthur LO, Ott DE, Lifson JD: Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and majorhistocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis andimmune regulation. J Virol. 2001 Jul;75(13):6173-82.
  Yamada T, Zhu D, Saxon A, Zhang K: CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase. J Biol Chem. 2002 Aug 9;277(32):28830-5., And many other.
  

Target Info

• Gene Symbol: PTPRC
• Gene Full Name: protein tyrosine phosphatase receptor type C
• Alias Symbols: B220;CD45;CD45 antigen;CD45R;GP180;LCA;L-CA;Leukocyte common antigen;LY5;protein tyrosine phosphatase, receptor type, c polypeptide;receptor-type tyrosine-protein phosphatase C;T200;T200 glycoprotein;T200 leukocyte common antigen.
• NCBI Gene Id: 5788
• Protein Name: Receptor-type tyrosine-protein phosphatase C
• Description of Target: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
• Uniprot ID: P08575
• Protein Accession #: NP_002829.3; NP_563578.2
• Nucleotide Accession #: NM_002838.4; NM_080921.3