PTPRC Antibody : APC-Cy7 (OATA00130)

Catalog No: OATA00130

Human peripheral blood lymphocytes were stained with 5 uL (0.25 ug) APC-Cyanine7 Anti-Human CD45RA (OATA00130) (solid line) or 0.25 ug APC-Cyanine7 Mouse IgG2b isotype control (dashed line).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Chimpanzee
• Product Format: Liquid 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: HI100
• Isotype: Mouse IgG2b, kappa
• Host: Mouse
• Conjugation: APC: Allophycocyanin, CY7: Cyanine 7
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 785 nm
• Reconstitution and Storage: 2-8°C protected from light. Expires 6 months from date of receipt. Do not freeze.
• Purification: Affinity chromatography
• Specificity: CD45RA
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Kroenke MA, Eto, D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow Cytometry)

  Lopez-Verges S, Milush JM, Schwartz BS, Pando MJ, Jarjoura J, York VA, Houchins JP, Miller S, Kang S-M, Norris PJ, Nixon DF, and Lanier LL. 2011. Proc. Natl. Acad. Sci. 108: 14725-14732. (Flow Cytometry)

  Imanguli MM, Swaim WD, League SC, Gress RE, Pavletic SZ, and Hakim FT. 2009. Blood. 113: 3620-3630. (Immunohistochemistry - paraffin embedded tissue)

  Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (Western Blot)

  Weninger W, Carlsen HS, Goodarzi M, Moazed F, Crowley MA, Baekkevold ES, Cavanagh LL, and von Andrian U. 2003. J. Immunol. 170: 4638-4648. (Immunohistochemistry - frozen tissue.

  Yamada T, Zhu D, Saxon A, and Zhang K. 2002. J. Biol. Chem. 277(32): 28830-28835. (in vitro blocking)

Target Info

• Gene Symbol: PTPRC
• Gene Full Name: protein tyrosine phosphatase receptor type C
• Alias Symbols: LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180
• NCBI Gene Id: 5788
• Protein Name: receptor-type tyrosine-protein phosphatase C
• Description of Target: The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cellspecific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others. The HI100 antibody is widely used as a marker for human CD45RA expression on naive and activated T cells, B cells, and monocytes.
• Uniprot ID: P08575
• Protein Accession #: NP_002829.3
• Nucleotide Accession #: NM_002838.4