Ptpn11 ELISA Kit (Rat) (OKCD01775)

Catalog No: OKCD01775 (Formerly GWB-KBBCX5)

Size:96WELLS

Rat Ptpn11 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ptpn11 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ptpn11 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Ptpn11 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.107 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PTPN11
• Gene Full Name: Protein tyrosine phosphatase, non-receptor type 11
• Alias Symbols: Shp2
• NCBI Gene Id: 25622
• Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• Description of Target: Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.
• Uniprot ID: P41499
• Protein Accession #: NP_001171064.1
• Nucleotide Accession #: NM_001177593.1
• Protein Size (# AA): 597