PTP4A2 Antibody - middle region (OAAB03940)

Catalog No: OAAB03940

Size:400ul

PTP4A2 Antibody (Center) (Cat. #OAAB03940) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PTP4A2 antibody detected the PTP4A2 protein (arrow).

Formalin-fixed and paraffin-embedded human prostate carcinoma with PTP4A2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence analysis of PTP4A2 Antibody (Center) with paraffin-embedded human prostate carcinoma tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Flow cytometric analysis of widr cells using PTP4A2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC, IF
  Description: This PTP4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38~68 amino acids from the Center region of human PTP4A2.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PTP4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-59 amino acids from the Central region of human PTP4A2.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PTP4A2 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
  IF: 1:10~50
• Other Applications Image 1 Data: Immunofluorescence analysis of PTP4A2 Antibody (Center) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterst
• Other Applications Image 2 Data: Flow cytometric analysis of widr cells using PTP4A2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: PTP4A2
• Alias Symbols: HH13, OV-1, PRL2, HH7-2, PRL-2, PTP4A, HU-PP-1, PTPCAAX2, ptp-IV1a, ptp-IV1b
• NCBI Gene Id: 8073
• Protein Name: Protein tyrosine phosphatase type IVA 2
• Uniprot ID: Q12974
• Protein Accession #: NP_001182029.1
• Molecular Weight: 19127 Da