Catalog No: OCOA10079
Size:20UG
Price: $179.00
SKU
OCOA10079
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PTOV1 Protein Lysate (OCOA10079)

Datasheets/ManualsPrintable datasheet for PTOV1 Protein Lysate (OCOA10079)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionPTOV1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolPTOV1
Gene Full NamePTOV1 extended AT-hook containing adaptor protein
Alias SymbolsACID2;activator interaction domain-containing protein 2;prostate tumor overexpressed 1;prostate tumor-overexpressed gene 1 protein;PTOV-1.
NCBI Gene Id53635
Protein NameNM_017432, NP_059128, Q86YD1
Description of TargetHomo sapiens prostate tumor overexpressed 1 (PTOV1), transcript variant 1, mRNA, Prostate tumor-overexpressed gene 1 protein, prostate tumor-overexpressed gene 1 protein isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q86YD1
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_059128
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_017432
Molecular Weight46.7 kDa
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