Catalog No: OKEH00937
Size:96T
Price: $525.00
SKU
OKEH00937
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PTN ELISA Kit (Human) : 96 Wells (OKEH00937)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PTN ELISA Kit (Human) (OKEH00937) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PTN captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
PTN Microplate96 Wells (12 x 8 Well strips)
PTN Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PTN Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Pleiotrophin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPTN
Gene Full Namepleiotrophin
Alias SymbolsHARP;HBBM;HB-GAM;HBGF8;HBGF-8;HBNF;HBNF-1;heparin affin regulatory protein;heparin-binding brain mitogen;heparin-binding growth factor 8;heparin-binding growth-associated molecule;heparin-binding neurite outgrowth promoting factor;heparin-binding neurite outgrowth-promoting factor 1;NEGF1;OSF-1;osteoblast-specific factor 1;pleiotrophin;pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1).
NCBI Gene Id5764
Protein NameP21246
Description of TargetPleiotrophin
Uniprot IDhttps://www.uniprot.org/uniprot/P21246
Protein Accession #NP_002816.1
Nucleotide Accession #NM_002825.5
Protein Size (# AA)168
Molecular Weight18 kDa
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