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PTN ELISA Kit (Human) : 96 Wells (OKEH00937)

96 Wells
$469.00
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Predicted Species Reactivity:
Human
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology PTN ELISA Kit (Human) (OKEH00937) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PTN captured in well.
Kit Range:
15.6 - 1000pg/mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
Kit Reproducibility:
Mean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 9.3% (n = 20)
Kit Component:
ComponentAmount
PTN Microplate96 Wells (12 x 8 Well strips)
PTN Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PTN Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
10 pg/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Human Pleiotrophin
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol:
PTN
Official Gene Full Name:
pleiotrophin
Alias Symbols:
HARP, HBBM, HB-GAM, HBGF8, HBGF-8, HBNF, HBNF1, HBNF-1, Heparin-binding brain mitogen, Heparin-binding growth-associated molecule, Heparin-binding growth factor 8, Heparin-binding neurite outgrowth-promoting factor 1, NEGF1, OSF-1, Osteoblast-specific fac
NCBI Gene Id:
5764
Protein Name:
Pleiotrophin
Swissprot Id:
P21246
Protein Accession #:
NP_002816.1
Nucleotide Accession #:
NM_002825.5
Protein Size (# AA):
168
Molecular Weight:
18 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PTN.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PTN.

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