PTN Chemi-Luminescent ELISA Kit (Rat) (OKCD03892)

Catalog No: OKCD03892

Size:96WELLS

Rat PTN ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-105%	91-98%	81-94%	84-104%
  EDTA Plasma (n=5)	79-99%	93-104%	79-95%	85-92%
  Heparin Plasma (n=5)	97-105%	97-104%	84-92%	90-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Pleiotrophin (PTN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pleiotrophin (PTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pleiotrophin (PTN) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	96
  EDTA Plasma (n=5)	96-103	101
  Heparin Plasma (n=5)	81-98	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Ptn Microplate: 96 Wells (12 x 8 Well strips)
  • Ptn Lyophilized Standard: 2
  • 100X Ptn Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ptn
• Gene Full Name: pleiotrophin
• Alias Symbols: HARP;HBBM;HB-GAM;HBGF-8;Hbnf;heparin affine regulatory peptide;heparin binding factor;heparin-binding brain mitogen;heparin-binding growth factor 8;heparin-binding growth-associated molecule;heparin-binding neurotrophic factor;heparin-binding neutrophic factor;OSF-1;osteoblast-specific factor 1;pleiotrophin;Pleiotrophin (Heparine binding factor, Hbnf, in the mouse).
• NCBI Gene Id: 24924
• Protein Name: Pleiotrophin
• Description of Target: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. Binds to cell-surface target proteins via their chondroitin sulfate groups (By similarity). Down-regulates PTPRZ1 activity (PubMed:16814777).
• Uniprot ID: P63090
• Protein Accession #: NP_058762
• Nucleotide Accession #: NM_017066