PTK7 ELISA Kit (Human) (OKCA01308)

Catalog No: OKCA01308

Size:96 Wells

Human PTK7 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	89	84-95
  1:2	92	87-101
  1:4	98	94-104
  1:8	98	93-105

• ELISA Kit Principle: Aviva Systems Biology PTK7 ELISA Kit (Human) (OKCA01308) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTK7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTK7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PTK7 captured in the well.
• ELISA Kit Range: 12.5 - 800 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	97	93-102
  EDTA Plasma (n=4)	91	84-97

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01308 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01308 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • PTK7 Microplate: 96 Wells (12 x 8 Well strips)
  • PTK7 Lyophilized Standard: 2 x 800 pg
  • 100X Biotinylated PTK7 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 3.125 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: PTK7
• Gene Full Name: protein tyrosine kinase 7 (inactive)
• Alias Symbols: CCK4, CCK-4
• NCBI Gene Id: 5754
• Protein Name: Inactive tyrosine-protein kinase 7
• Description of Target: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
• Uniprot ID: Q13308
• Protein Accession #: NP_001257327.1
• Nucleotide Accession #: NM_001270398.1