PTK2B Antibody (Phospho-Tyr579) (OASG06142)

Catalog No: OASG06142

Size:100 uL

Western Blot using 3T3

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human PYK2 around the phosphorylation site of Y579. Location: 520-600aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Tyr579
• Specificity: Phospho-PYK2 (Tyr579) Polyclonal Antibody detects endogenous levels of PYK2 protein only when phosphorylated at Y579.
• Application Info: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Target Info

• Gene Symbol: PTK2B
• Gene Full Name: protein tyrosine kinase 2 beta
• Alias Symbols: PKB, PTK, CAKB, FAK2, PYK2, CADTK, FADK2, RAFTK
• NCBI Gene Id: 2185; 19229; 50646
• Description of Target: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
• Uniprot ID: Q14289, Q9QVP9, P70600
• Molecular Weight: 116 kDa