PTK2B Antibody (OAGA08687)

Catalog No: OAGA08687

Size:100 ul

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody [GT858] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: GT858
• Isotype: IgG2a
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of Human PYK2. The exact sequence is proprietary.
• Purification: Affinity purified by Protein A.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: PBS

Target Info

• Gene Symbol: PTK2B
• Gene Full Name: protein tyrosine kinase 2 beta
• Alias Symbols: PKB, PTK, CAKB, FAK2, PYK2, CADTK, FADK2, RAFTK
• NCBI Gene Id: 2185
• Protein Name: protein-tyrosine kinase 2-beta
• Description of Target: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
• Uniprot ID: Q14289
• Protein Accession #: NP_004094.3
• Nucleotide Accession #: NM_004103.4