Catalog No: OCOA03886
Size:20UG
Price: $179.00
SKU
OCOA03886
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PTHLH Protein Lysate (OCOA03886)

Datasheets/ManualsPrintable datasheet for PTHLH Protein Lysate (OCOA03886)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionPTHLH HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolPTHLH
Gene Full Nameparathyroid hormone like hormone
Alias SymbolsBDE2;HHM;osteostatin;parathyroid hormone-like hormone preproprotein;Parathyroid hormone-like protein;parathyroid hormone-like related protein;parathyroid hormone-related protein;PLP;PTHR;PTH-related protein;PTH-rP;PTHRP.
NCBI Gene Id5744
Protein NameA0A024RB29, NM_198965, NP_945316, P12272
Description of TargetHomo sapiens parathyroid hormone like hormone (PTHLH), transcript variant 1, mRNA, Parathyroid hormone-related protein, parathyroid hormone-related protein isoform 1 preproprotein [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P12272
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_945316
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_198965
Molecular Weight16 kDa
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