PTHLH ELISA Kit (Rat) (OKCA01915)

Catalog No: OKCA01915

Size:96 Wells

Rat PTHLH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology PTHLH ELISA Kit (Rat) (OKCA01915) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PTHLH has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PTHLH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PTHLH captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	104	99-107
  EDTA Plasma (n=4)	92	86-96

• ELISA Kit Component:
  • PTHLH Microplate: 96 Wells (12 x 8 Well strips)
  • PTHLH Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PTHLH Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: PTHLH
• Gene Full Name: Parathyroid hormone-related protein
• Alias Symbols: PTHRP, PTH-rP
• NCBI Gene Id: 100009357
• Protein Name: Parathyroid hormone-related protein
• Description of Target: Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath.Osteostatin is a potent inhibitor of osteoclastic bone resorption.
• Uniprot ID: Q9GLC7
• Protein Accession #: NP_001076122.1
• Nucleotide Accession #: NM_001082653.1