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PTH ELISA Kit (Rat) (OKEH03237)

96 Wells
$529.00
In Stock
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Gene Symbol:
PTH
Official Gene Full Name:
parathyroid hormone
NCBI Gene Id:
24694
Alias Symbols:
Parathyrin, Parathyroid hormone, PTH, PTH-(1-84), Pth1, Pthr1
Description of Target:
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Protein Name:
Parathyroid hormone(PTH)
Swissprot Id:
P04089
Protein Accession #:
NP_058740.1
Nucleotide Accession #:
NM_017044.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 23.4 pg/mL
Kit Range:
23.4 - 1500 pg/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology PTH ELISA Kit (Rat) (OKEH03237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pth has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pth is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pth captured in well.
Kit Component:
ComponentAmount
Anti-Pth Microplate96 Wells (12 x 8 Well strips)
Pth Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Pth Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Pth.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Pth.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural ratPTH. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
13 kDa
Protein Size (# AA):
115

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