Size:96 Wells
$469.00
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SKU
OKEH03237
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PTH ELISA Kit (Rat) (OKEH03237)

Catalog#: OKEH03237
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityRat
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology PTH ELISA Kit (Rat) (OKEH03237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pth has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pth is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pth captured in well.
Kit Range23.4 - 1500pg/mL
Kit Component
ComponentAmount
Anti-Pth Microplate96 Wells (12 x 8 Well strips)
Pth Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Pth Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity11.7 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Rat Parathyroid hormone
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPTH
Official Gene Full Nameparathyroid hormone
Alias SymbolsParathyrin, Parathyroid hormone, PTH, PTH-(1-84), Pth1, Pthr1
NCBI Gene Id24694
Protein NameParathyroid hormone(PTH)
Description of TargetPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Swissprot IdP04089
Protein Accession #NP_058740.1
Nucleotide Accession #NM_017044.1
Protein Size (# AA)115
Molecular Weight13 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Pth.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Pth.
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