PTGER2 ELISA Kit (Human) (OKCD01186)

Catalog No: OKCD01186 (Formerly GWB-KBBDW7)

Size:96WELLS

Human PTGER2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	79-101%	92-105%	94-102%	81-91%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin E Receptor 2 (EP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin E Receptor 2 (EP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin E Receptor 2 (EP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prostaglandin E Receptor 2 (EP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	91-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin E Receptor 2 (EP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin E Receptor 2 (EP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PTGER2 Microplate: 96 Wells (12 x 8 Well Strips)
  • PTGER2 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated PTGER2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 29 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prostaglandin E Receptor 2 (EP2). No significant cross-reactivity or interference between Prostaglandin E Receptor 2 (EP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PTGER2
• Gene Full Name: prostaglandin E receptor 2
• Alias Symbols: EP2;PGE receptor EP2 subtype;PGE2 receptor EP2 subtype;prostaglandin E receptor 2 (subtype EP2), 53kD;prostaglandin E receptor 2 (subtype EP2), 53kDa;prostaglandin E2 receptor EP2 subtype;prostanoid EP2 receptor.
• NCBI Gene Id: 5732
• Protein Name: Prostaglandin E2 receptor EP2 subtype
• Description of Target: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
• Uniprot ID: P43116
• Protein Accession #: NP_000947
• Nucleotide Accession #: NM_000956.3
• Protein Size (# AA): 358