PTGDS ELISA Kit (Mouse) (OKCA02434)

Catalog No: OKCA02434

Size:96 Wells

Mouse PTGDS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	93	88-97
  1:2	84	80-88
  1:4	95	90-100
  1:8	98	94-105

• ELISA Kit Principle: Aviva Systems Biology Ptgds ELISA Kit (Mouse) (OKCA02434) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptgds has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ptgds is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Ptgds captured in the well.
• ELISA Kit Range: 4.7 - 300 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	100	95-109
  EDTA Plasma (n=4)	92	87-96

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Ptgds Microplate: 96 Wells (12 x 8 Well strips)
  • Ptgds Lyophilized Standard: 2
  • 100X Biotinylated Ptgds Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.2 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PTGDS
• Alias Symbols: L-P, PGD, Ptg, PGD2, PGDS, 21kDa, PGDS2, Ptgs3, L-PGDS
• NCBI Gene Id: 19215
• Protein Name: Prostaglandin-H2 D-isomerase
• Description of Target: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
• Uniprot ID: O09114
• Protein Accession #: NP_032989
• Nucleotide Accession #: NM_008963